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  Product  (Z - AA)2Rh110[Rhodamine 110
  bis - (CBZ - L - alanyl - L - alanine amide)]
  CAS  
  Size  5 mg
  Catalog #  60320-5
  US$  115
  Hits  

Description

(Z-Ala-Ala)2Rh110 is a very sensitive fluorogenic calpain substrate. In general, Rh110-based protease substrates are much more sensitive than the analogous AMC/AFC-based substrate. This enhanced sensitivity might be attributed to the greater fluorescence of the enzymatic product. The colorless and nonfluorescent (Z-Ala-Ala)2Rh110 is hydrolyzed to highly fluorescent rhodamine 110, which exhibits excellent spectral properties that match the optimal detection window of most fluorescence instruments. Alternatively, (Z-Ala-Ala)2Rh110 can also be used to detect calpain in a chromogenic mode since the enzymatic product (rhodamine 110) exhibits a large extinction coefficient (close to 100,000 cm-1mol-1).

Minimum Purity: 95%

Storage -20C desiccated and protected from light
References Gitler D and Spira ME (1998). Real time imaging of calcium-induced localized proteolytic activity after axotomy and its relation to growth cone formation. Neuron 20, 1123-35, Rothe G, et al. (1992). Flow cytometric analysis of protease activities in vital cells. Biol Chem Hoppe Seyler 373, 547-54, Rothe G, et al. (1992). Flow cytometric analysis of protease activities in vital cells. Biol Chem Hoppe Seyler 373, 547-54.
Molecular Weight 885.6
Spectral Properties Abs/Em = 497/520 nm
Solvent System DMSO


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